Improved Detection of Myositis-Specific Autoantibodies Using Luciferase Immunoprecipitation Systems Assay: Comparison with Line Blot and Conventional Immunoprecipitation - PubMed
6 hours ago
- #immunoassay
- #autoantibodies
- #myositis
- The study compares the performance of the luciferase immunoprecipitation systems (LIPS) assay with line blot and conventional immunoprecipitation (IP) for detecting myositis-specific autoantibodies.
- LIPS demonstrated excellent agreement with IP (κ=0.90, AUC 0.935) and outperformed line blot in accuracy.
- Line blot performance varied with thresholds: ≥36 AU increased sensitivity but reduced specificity, while ≥71 AU improved specificity but lowered sensitivity.
- Antibody-specific thresholds modified line blot concordance but were quantitatively inferior to LIPS.
- LIPS-positive cases were more frequently associated with antisynthetase syndrome and dermatomyositis.
- Discordant cases (line blot-positive/LIPS-negative) were often clinically classified as inclusion body myositis.
- LIPS provides quantitative discrimination and reduces misclassification, enhancing clinical stratification in myositis patients.