Roles of microtubules and LIS1 in dynein transport machinery assembly - PubMed
4 days ago
- #LIS1
- #dynein
- #microtubules
- Cytoplasmic dynein-1 requires dynactin and a coiled-coil adaptor to form the dynein-dynactin-adaptor (DDA) complex.
- Microtubules (MTs) facilitate the spontaneous formation of an adaptor-independent dynein-dynactin complex with a 2:1 stoichiometry.
- Adaptors can integrate into and exchange within the MT-bound dynein-dynactin complex, aided by dynein light-intermediate chains.
- LIS1 is not essential for DD(A)-MT assembly but expands the conformational landscape of DD(A) assemblies on MTs.
- LIS1 bridges dynactin p150glued and dynein, stabilizing low-MT-affinity intermediates and priming dynein for DDA assembly.
- The study highlights the dynamic adaptability of the dynein transport machinery and the coordinated roles of MTs and LIS1 in DDA assembly.