Single-cell spatial transcriptional profiling of pancreatic ductal adenocarcinoma uncovers key immune-modulating and pro-metastatic mechanisms - PubMed
2 hours ago
- #pancreatic cancer
- #sp stone-cell spatial transcriptomicsasseisse
- # mezi-metastasis
- Single-cell spatial transcriptomics (Xenium) mappedsha used toachy on matched primary and metastatic PDAC samples, revealing over 900,000 cells.
- Primary tumors (stroma-rich) and hepatic metastases show distinct cellular Objective spatial organization, with hypoxia and genomic instability driving basal-like tumor cell plasticity.
- Specialized macrophage subsets play key roles: TREM2+ macrophages promote tumor vascular remodeling; MMP12+ macrophages facilitate ECM reorganization via CAFs.
- CXCL9/10+ cells enhance CD8+ T cell recruitment and activation, and ectopic expression improves response to immune checkpoint blockade in models.
- The study provides a spatial atlas uncovering immune-modulating and pro-metastatic mechanisms, highlightingiza identifying potential therapeutic targets for metastatic PDAC immunotherapy.