The interplay between Peptostreptococcus and Fusobacterium as novel signatures in colorectal cancer recurrence - PubMed
5 hours ago
- #recurrence
- #colorectal-cancer
- #microbiome
- More than 30% of colorectal cancer (CRC) patients experience recurrence after resection surgery.
- The study analyzed 120 tumor mucosal samples using 16S rRNA gene sequencing and LC-MS metabolomic profiling.
- Recurrent patients showed distinct microbial and metabolomic signatures compared to non-recurrent patients.
- An integrated signature of five bacterial genera and five metabolites was identified with strong discriminatory performance (AUC = 0.89).
- High-risk patients had significantly shorter recurrence-free survival (adjusted HR = 1.59, P < 0.0001).
- Fusobacterium and Peptostreptococcus were found to co-aggregate and form dense dual-species biofilms.
- P. anaerobius enhanced F. nucleatum adhesion to tumor cells and colonization of colonic mucosa.
- Arginine and proline metabolism pathways were enriched in the recurrence group.
- Arginine disrupted F. nucleatum-P. anaerobius co-aggregation, while putrescine promoted dual-species biofilm formation.
- The study proposes a risk stratification framework and highlights a link between pathobionts and metabolites relevant to recurrence.