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A pangenome reference and population studies link structural variants with breeding traits in Gossypium hirsutum - PubMed

5 hours ago
  • #cotton
  • #breeding
  • #genomics
  • A telomere-to-telomere (T2T) genome was assembled for the elite cotton cultivar NDM13.
  • Near-T2T genomes were also assembled for 27 other Gossypium hirsutum representatives.
  • Transcriptomic profiling was conducted on 15 distinct tissues from each cultivar.
  • 51,551 conserved orthologs were identified across all genomes.
  • Structural variations (SVs), segmental duplications, and copy number variants were analyzed for their impact on gene expression and adversity resistances.
  • Divergent SVs and genes linked to modern breeding evolution were identified.
  • A pangenome was constructed using T2T-reference and 761,536 SVs from 1,671 worldwide accessions.
  • Hidden SVs influencing critical breeding traits were discovered.
  • The findings are expected to enhance genetic studies and biotechnological improvements in cotton.