Spatial Transcriptomics Characterisation of Radionecrotic Changes in Glioblastoma Patients - PubMed
12 hours ago
- #radionecrosis
- #glioblastoma
- #spatial transcriptomics
- IDH-wildtype Glioblastoma (GB) is the most common primary malignant CNS tumor in adults.
- Standard treatment includes radiotherapy, which can lead to radionecrotic changes as a late complication.
- Differentiating between tumor progression and radionecrotic changes is challenging due to similar MRI appearances and symptoms.
- Histopathological examination remains the gold standard for diagnosis.
- Spatial single-cell transcriptomic profiling was performed on samples from GB patients using the Xenium platform.
- Progressive GB samples showed OPC/NPC-like and proliferating tumor cells with high EGFR expression.
- Radionecrotic samples had tumor cells with downregulated EGFR expression, even with gene amplification, and lacked proliferation markers.
- Border-associated macrophages infiltrated radionecrotic tissue, potentially promoting gliosis.
- The study provides a spatial transcriptomic atlas of GB progression and radionecrotic changes, aiding future research.