Understanding and addressing oocyte maturation failure and failed or abnormal fertilization during oocyte-to-zygote transition - PubMed
5 hours ago
- #fertilization failure
- #oocyte maturation
- #zygote rescue
- The transition from immature oocyte to fertilized zygote is critical, with failures possible during maturation or fertilization.
- In assisted reproduction, oocytes at prophase I or metaphase I (MI) can be cultured to reach metaphase II (MII), but current culture systems limit maturation rates and developmental potential.
- Fertilization failure can occur; failed fertilized oocytes may be treated with ICSI within 24 hours, yielding sub-optimal but clinically significant outcomes.
- Atypical fertilization can produce monopronuclear (1PN) or tripronuclear (3PN) zygotes, with 1PN zygotes potentially being biparental diploid and suitable for clinical use after blastocyst development and chromosomal testing.
- Rescue techniques for oocytes and zygotes are gaining interest, especially when additional embryos could impact clinical outcomes.