Expansion and CAR engineering of granulocyte-monocyte progenitors for cellular immunotherapy - PubMed
5 hours ago
- #cellular immunotherapy
- #CAR engineering
- #GMP expansion
- Developed defined culture conditions enabling long-term expansion of mouse and human granulocyte-monocyte progenitors (GMPs) while preserving their identity and potential.
- Identified myeloperoxidase as a regulator of GMP proliferation, establishing GMPs as a renewable platform for engineering.
- Engineered GMPs seeded hematopoietic niches after transfer, generating donor-derived myelopoiesis that restored antibacterial defense and produced tumor-infiltrating macrophages.
- GMPs engineered with chimeric antigen receptors (CARs) suppressed CD19-positive leukemia and HER2-positive solid tumors effectively.
- Introduced a CAR with an IgG Fc domain that recruits host phagocytes, enables T cell priming across MHC mismatch, and enhances efficacy in immunocompetent allogeneic cancer models.