Anti-Inflammatory and Angiogenic Effects of Stem Cell Secretome - PubMed
6 hours ago
- #stem cell secretome
- #extracellular vesicles
- #osteoarthritis
- The study focuses on the anti-inflammatory and angiogenic effects of stem cell secretome, particularly from human embryonic stem cell-derived mesenchymal stem cells (ES-MSCs) and Infrapatellar fat pad derived MSCs (IPFP-MSC).
- Secretome and extracellular vesicles (EVs) were isolated and characterized, showing similar particle size distribution (50-200 nm) and expression of exosomal markers (CD9, CD63, CD81).
- Concentrated conditioned medium (CCM) and EVs enhanced cell proliferation in a dose-dependent manner and stabilized endothelial network formation in fibrin gels over 7 days.
- In an osteoarthritic (OA) cartilage model, ES-MSC CCM and EV treatments reduced glycosaminoglycan release and decreased expression of catabolic genes (IL-1β, MMP-1, MMP-3).
- The findings highlight the therapeutic potential of stem cell-derived secretomes for osteoarthritis, promoting cell proliferation, angiogenesis, and mitigating cartilage degradation.