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Single-cell Transcriptomics Reveals Cytotrophoblast Subtypes and Senescence in Recurrent Spontaneous Abortion - PubMed

7 hours ago
  • #placental dysfunction
  • #single-cell RNA sequencing
  • #cellular senescence
  • Recurrent spontaneous abortion (RSA) involves the loss of two or more pregnancies before 20 weeks of gestation.
  • Single-cell RNA sequencing identified three cytotrophoblast (CTB) subtypes in RSA: CTB1 (protein synthesis), CTB2 (proliferative progenitor), and CTB3 (adhesive/invasive precursor to EVTs).
  • Syncytiotrophoblasts (STBs) were characterized for hormone production, with impaired function in RSA.
  • CTB2 and CTB3 in RSA exhibited cellular senescence, reducing proliferative and differentiation capacity.
  • Aberrant trophoblast senescence may contribute to defective placentation in RSA, suggesting potential therapeutic targets.