Unravelling chaperone-mediated microautophagy targeting KFERQ-like motif containing proteins in yeast - PubMed
7 hours ago
- #Microautophagy
- #Chaperone-mediated autophagy
- #KFERQ motif
- Chaperone-mediated autophagy (CMA) in mammals degrades proteins with KFERQ-like motifs during prolonged starvation using LAMP2A as a receptor.
- Saccharomyces cerevisiae lacking LAMP2A still translocates proteins with these motifs into vacuoles under prolonged starvation.
- Phosphatidylserine on the vacuolar membrane acts as a receptor for Hsp70-substrate complexes, facilitating protein translocation.
- This newly identified pathway is termed chaperone-mediated microautophagy, relying on cytosolic Hsp70 and the ESCRT complex, involving vacuolar membrane invagination.
- The discovery highlights a novel cellular pathway in yeast for clearing proteins under chronic stress conditions.