Identification and Validation of miR-206-3p Targeting WT-1 Promotes Membranous Nephropathy Through a Comprehensive Bioinformatics and Machine Learning Algorithm - PubMed
7 hours ago
- #miR-206-3p
- #bioinformatics
- #membranous nephropathy
- Study identifies miR-206-3p targeting WT-1 as a key mechanism in membranous nephropathy (MN).
- Utilized bioinformatics, machine learning, and validation methods to analyze MN biomarkers.
- Found 13 upregulated differentially expressed miRs (DEmiRs) and 58 miRs correlated with MN via WGCNA.
- LASSO method narrowed down to 5 candidate hub miRs, with miR-206 and WT-1 emerging as key biomarkers.
- Validated findings in passive Heymann nephropathy rat model and adriamycin-injured podocytes.
- Confirmed miR-206-3p upregulation and WT-1 downregulation in apoptotic podocytes in vivo and in vitro.
- Identified C-MIP/Nephrin/PI3K pathway as potential mechanism for miR-206-3p targeting WT-1 in podocyte apoptosis.
- Inhibition of miR-206-3p restored WT-1 expression and reduced podocyte apoptosis.