Modeling mitochondrial inheritance enables high-precision single-cell lineage tracing in humans - PubMed
4 hours ago
- #lineage tracing
- #mitochondrial DNA
- #single-cell analysis
- MitoDrift is a probabilistic framework for high-precision lineage tracing using mitochondrial DNA (mtDNA) mutations.
- It integrates Wright-Fisher drift dynamics with sparse single-cell measurements to refine lineage trees.
- Validation shows MitoDrift outperforms existing methods in precision and clonal recovery.
- Applied to human hematopoiesis, it reveals age-associated decline in clonal diversity and heritable regulatory programs.
- In multiple myeloma, MitoDrift detects therapy-associated clonal remodeling and links gene regulatory programs to drug sensitivity.
- Enables quantitative lineage-state analysis in human tissues, connecting clonal history to transcriptional and epigenetic programs.