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Spatial transcriptomics from pancreas and local draining lymph node tissue reveals a lymphotoxin-β signature in human type 1 diabetes - PubMed

5 hours ago
  • #lymphotoxin-β
  • #type 1 diabetes
  • #spatial transcriptomics
  • Study explores inflammatory response in pancreas and pancreatic lymph nodes (pLNs) during type 1 diabetes (T1D) progression.
  • Spatial transcriptomics (ST) used to profile non-diabetic (ND), at-risk autoantibody-positive (AAb+), and T1D donors.
  • T1D pancreas shows global upregulation of inflammation-associated transcripts (REG family, C3, SOD2, OLFM4).
  • T1D pLN exhibits significant upregulation of LTB within lymphoid follicles.
  • Follicular B cells identified as primary source of LTB in pLN using subcellular-resolution ST.
  • Increased LTB expression observed in lymphocytes near CCL19/CCL21-expressing endothelium in insulitic lesions.
  • Findings highlight lymphotoxin-β and chemokine signatures in pancreatic lymphatics and insulitic lesions.
  • Results may inform future therapeutic interventions for T1D.