NAT10-mediated ac4C RNA acetylation stabilizes CXCL5/DEK mRNA to drive proliferation and metastasis in lung adenocarcinoma - PubMed
5 hours ago
- #Lung adenocarcinoma
- #NAT10
- #RNA acetylation
- NAT10-mediated ac4C RNA acetylation stabilizes CXCL5/DEK mRNA, driving proliferation and metastasis in lung adenocarcinoma (LUAD).
- NAT10 expression is significantly upregulated in LUAD tissues and cell lines, confirmed by bioinformatics and experimental data.
- CRISPR/Cas9 knockout of NAT10, followed by acRIP-seq and RNA-seq, revealed altered expression of CXCL5 and DEK genes, linked to the adhesion signaling pathway.
- NAT10 acts as an ac4C 'writer', enhancing mRNA stability of CXCL5 and DEK by acetylation, thus preventing their degradation.
- NAT10 significantly influences metastasis and tumor growth in mouse models, suggesting its potential as a diagnostic biomarker or therapeutic target in LUAD.
- The study complies with ethical standards, with samples obtained from the Second Affiliated Hospital of Harbin Medical University under institutional approval.