Transcription factor NF-Y complex interacts with chromatin remodeling complexes SWI/SNF and RSC to coordinately regulate gene expression - PubMed
4 hours ago
- #gene regulation
- #chromatin remodeling
- #transcription factor
- The NF-Y complex is an evolutionarily conserved transcription factor that binds the CCAAT box in about 30% of eukaryotic promoters.
- In fungi Penicillium oxalicum and Trichoderma reesei, NF-Y subunits captured near-complete chromatin remodeling complexes (CRCs) SWI/SNF and RSC, revealing four novel filamentous-fungi-specific subunits.
- SWI/SNF subunits (Snf19/Sol1/Fif1) and RSC subunits (Rsc7/Fif3) serve as direct interaction targets for NF-Y, enabling coordination between NF-Y and the CRCs.
- Functional studies in P. oxalicum show overlapping but distinct roles for NF-Y subunits (PoNF-YB, PoNF-YC) and CRC-specific subunits (PoFif1, PoFif3) in growth, development, and enzyme production.
- Chromatin occupancy of PoFif1 and PoFif3 at certain promoters depends on PoNF-Y, suggesting a model where NF-Y interacts with SWI/SNF and RSC to coordinately regulate gene expression.
- This interaction may explain how NF-Y, a canonical transcription factor, exhibits 'pseudo-chromatin remodeler' activity by engaging with authentic chromatin remodeling complexes.