Single-cell dissection of pleural and systemic immunity uncovers pathogen-specific immune reprogramming in tuberculosis versus lung adenocarcinoma - PubMed
5 hours ago
- #tuberculosis
- #immunology
- #single-cell sequencing
- Single-cell transcriptome analysis and TCR clonotype tracking were used to compare immune responses in lung adenocarcinoma (LUAD) and tuberculosis (TB).
- Th1/17 polarized pro-inflammation dominated TB effusions, while recently activated CD4 T cells in LUAD adopted an antitumor trajectory but were suppressed.
- CD8 T cells diverged into GZMH (antitumor killer cells suppressed in LUAD) and GZMK (stem-like precursors) subsets.
- MAITs and class-switched B cells showed higher activation and expansion in TB.
- Myeloid cells were more activated in TB, upregulating pro-inflammatory cytokines, while LUAD featured immunosuppressive signals.
- Cell-cell communication in LUAD highlighted outgoing interactions by myeloid cells (monocytes, DCs) via IL16 and VISFATIN signaling, correlating with survival.
- Distinct immune landscapes were observed in pleural effusions and peripheral blood from TB and LUAD patients.