A high-throughput conditioned-media-based screening system identifies inhibitors of aggregation induced by iPSC-secreted amyloid β - PubMed
8 hours ago
- #High-Throughput Screening
- #Alzheimer's Disease
- #Drug Discovery
- A high-throughput screening system named HaiDap was developed to identify inhibitors of amyloid β aggregation induced by iPSC-secreted proteins.
- The system uses conditioned media from differentiated Alzheimer's disease patient iPSCs, improving specificity over traditional PBS-based assays.
- Screening of 22 edible plant extracts identified three (O. aristatus, S. aromaticum, G. yesoense) that significantly delay Aβ aggregation in iPSC-based neuronal assays.
- The HaiDap system offers efficient, accurate, and low-cost screening for amyloid aggregation inhibitors, addressing limitations of conventional in vitro methods.