Small molecule-directed differentiation of submerged-cultured human nasal airway epithelia for respiratory disease modeling - PubMed
5 hours ago
- #disease modeling
- #epithelial differentiation
- #respiratory research
- Submerged cultures of undifferentiated or transformed epithelial cells are widely used in respiratory research due to their ease of use and scalability.
- A new submerged differentiation model using cryopreserved human nasal epithelial cells obtained via minimally invasive brushings is described.
- By targeting Notch and BMP signaling with small molecule inhibitors, cells are differentiated into complex epithelial cultures containing basal, secretory, and ciliated cell types.
- This method supports scalable culture of both 2D epithelial monolayers and 3D organoids.
- Applied to disease modeling in primary ciliary dyskinesia, cystic fibrosis, and respiratory syncytial virus infection.
- Enables scalable assessment of disease-relevant epithelial functions in respiratory research.