An inducible multiciliated cell line resolves proteome dynamics and identifies CDK7 as a conserved regulator - PubMed
4 days ago
- #Proteomics
- #Multiciliated cells
- #CDK7
- Multiciliated cells (MCCs) are essential for directional fluid flow in vertebrate organs.
- MCC differentiation involves tightly regulated centriole amplification.
- A stable inducible MCC line was derived from Xenopus A6 kidney epithelial cells.
- Upon induction of multicilin (MCI), A6-MCI cells differentiate into mature MCCs in 48 hours.
- Xenopus deuterosomes were characterized using custom antibodies and super-resolution imaging.
- Proteomic profiling identified CDK7 as a critical regulator in MCC differentiation.
- The study provides a resource for MCC biology and potential therapeutic targets for ciliopathies.