Reconstruction of T cell infiltration in an osteosarcoma PDX-organoid interactive biobank for personalized immunotherapy - PubMed
5 hours ago
- #organoid
- #precision therapy
- #osteosarcoma
- Osteosarcoma (OS) is an aggressive malignant bone tumor with limited therapeutic options and low response to immunotherapy.
- An OS PDX-organoid interactive biobank was established by self-assembling single-cell suspensions into individualized OS organoids (iOSs).
- iOS models recapitulate OS spatial and architectural features at millimeter scale in vitro and as xenografts.
- iOS models maintain functional pairing with matched PDX models.
- iOS fidelity was validated using histopathology, spatial features, genomics, transcriptomics, and pharmacogenomics.
- T cell infiltration was reconstructed in PDX-derived iOS models to model treatment-associated immune responses and support immunotherapy translational studies.
- A PRMT5MTA inhibitor enhances immunotherapy response in chromosome 9p21.3-deleted OS, demonstrated using paired iOS-PDX models.