Multiple pathways of CD34+ cell differentiation during embryogenesis - PubMed
5 hours ago
- #Embryonic Development
- #CD34+ cells
- #Lineage Tracing
- CD34 is a marker for endothelial progenitors and hematopoietic stem cells, involved in vascular development and hematopoiesis.
- Inducible genetic lineage tracing, proteomics, and scRNA-seq revealed dynamic developmental trajectories of CD34+ cells in human and mouse embryos.
- CD34+ cells give rise to distinct progenitor waves with endothelial, hematopoietic, and fibroblastic fates at different embryonic stages.
- During gastrulation (E6.5-E8.5), CD34+ progenitors primarily drive vasculogenesis via a Kdr-dependent mechanism.
- From E9.5 to E14.5, cell cycle activation facilitates the endothelial-to-hematopoietic transition (EHT) of CD34+ progenitors.
- A late embryonic wave of CD34+ progenitors generates fibroblasts, separate from earlier endothelial or hematopoietic lineages.
- In human cord blood, GFPT2 specifically marks fibroblast progenitors, distinguishing them from circulating endothelial progenitors.
- The study provides a high-resolution spatiotemporal atlas of CD34+ cells, offering insights for regenerative medicine applications.