Application of spatial transcriptomics across organoids for a high-resolution, spatial whole-transcriptome benchmarking dataset - PubMed
3 hours ago
- #organoids
- #benchmarking
- #spatial transcriptomics
- This study presents the first systematic spatial transcriptomic profiling of multiple stem cell-derived organoid models using stereo-seq.
- It describes assay optimization for characterizing organoids and assessing RNA capture efficiency compared to reference tissues.
- A bespoke analysis method is introduced to partition samples into regions for detailed characterization.
- The findings provide insights for future spatial transcriptomics studies on organoids, including optimization of RNA capture and regional analysis.