Engineering the ADDomer Nanoparticle Vaccine Scaffold for Improved Assembly and Enhanced Stability - PubMed
6 hours ago
- #Nanoparticle Vaccines
- #Protein Engineering
- #Virus-like Particles
- The ADDomer is a synthetic dodecahedral VLP scaffold derived from human adenovirus serotype 3 (Ad3) penton base protein (PBP).
- Two engineering strategies were employed: using a Chimpanzee adenovirus Y25-based ADDomer (CHIMPSELS) to avoid preexisting immunity and introducing intersubunit disulfide bonds for stability.
- High-resolution electron cryo-microscopy confirmed the formation of stable dodecahedral particles with disulfide bonds stabilizing PBP N-termini conformations.
- Disulfide-stabilized ADDomers showed improved thermal stability and higher aggregation onset temperatures, useful for vaccine development screening.
- The modified scaffolds successfully displayed immunogenic epitopes, including SARS-CoV-2 and Chikungunya peptides, maintaining structural integrity.
- The study presents a versatile, thermostable VLP platform with reduced preexisting immunity susceptibility and modular epitope presentation capacity.