Application of Intracellular Vesicles Surpass Extracellular Vesicles in Regenerative Repair of Inflammatory Bone Defects via the LAMA4-Mediated YAP/TAZ Signalling - PubMed
5 hours ago
- #bone regeneration
- #YAP/TAZ signaling
- #intracellular vesicles
- Intracellular vesicles (IVs) from human periodontal ligament stem cells (hPDLSCs) were produced with 6.9-fold higher efficiency and showed better cellular uptake than extracellular vesicles (EVs).
- Under LPS-induced inflammatory conditions, IVs outperformed EVs in enhancing hPDLSC proliferation, reducing pro-inflammatory cytokines (IL-6, TNF-α), and upregulating osteogenic markers via YAP/TAZ activation.
- Proteomic analysis identified laminin subunit alpha 4 (LAMA4) as a key factor enriched in IVs, essential for activating YAP/TAZ signaling and promoting osteogenesis.
- In vivo, IVs-loaded gel effectively promoted bone regeneration in rat periodontal defects without toxicity, positioning IVs as a high-yield cell-free therapy.
- The study highlights IVs as superior to EVs for regenerative repair in inflammatory bone defects, mediated by LAMA4-dependent YAP/TAZ signaling.