A Dual-Strategy Hydrogel Targeting Glycolytic Reprogramming and Inflammation to Inhibit Ferroptosis and Promote Intervertebral Disc Repair - PubMed
6 hours ago
- #hydrogel
- #glycolysis
- #ferroptosis
- Intervertebral disc degeneration (IVDD) is a major cause of lower back pain, driven by metabolic changes like enhanced glycolysis.
- Lactate, a glycolysis by-product, induces ferroptosis in nucleus pulposus cells (NPCs), worsening IVDD.
- Inflammation enhances glycolysis and upregulates lactate dehydrogenase A (LDHA), creating a vicious cycle.
- A dual-strategy hydrogel system was designed to target glycolytic reprogramming and inflammation.
- The hydrogel uses PBA-functionalized G5 PAMAM to encapsulate LDHA siRNA, forming self-assembled nanoparticles (GPS).
- GPS was modified with epigallocatechin (EGC) to form stable GPS-EGC nanoparticles via borate ester bonds.
- The nanoparticles are incorporated into a ROS- and pH-responsive hydrogel for controlled drug release.
- The system reduces IL-1β-induced inflammation and lactate production, alleviating ferroptosis in NPCs.
- This approach promotes tissue repair in IVDD models, offering a promising therapeutic strategy.
- The study highlights the potential of material innovation in modulating metabolic and inflammatory pathways.