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A conserved Arf-GEF modulates axonal integrity through RAB-35 by altering neuron-epidermal attachment - PubMed

5 hours ago
  • #Axonal Integrity
  • #AGEF-1
  • #RAB-35
  • The study investigates how neuron-epidermal attachments maintain axonal integrity under mechanical strain in C. elegans.
  • UNC-70/β-spectrin and RAB-35 work synergistically in the skin to stabilize these attachments and prevent axon damage.
  • A genetic screen identified AGEF-1, a guanine nucleotide exchange factor (GEF), as key for axonal maintenance in mechanosensitive neurons.
  • AGEF-1 acts selectively within the skin, binds to epidermal RAB-35, and regulates its activity to modulate attachment stability.
  • The human ortholog BIG2 can functionally replace AGEF-1, indicating high conservation of this molecular machinery.