MalDeepSeq panel: A targeted ultra-deep sequencing approach to trace drug resistance markers in Plasmodium falciparum - PubMed
4 hours ago
- #malaria
- #drug resistance
- #sequencing
- The study introduces a targeted ultra-deep sequencing panel for tracking drug resistance in Plasmodium falciparum.
- It covers full-length sequences of 48 antimalarial resistance genes with high performance in mock mixtures and clinical samples.
- Mutect2 and HaplotypeCaller were evaluated, with Mutect2 showing lower bias and error in variant detection.
- The panel effectively captures minority resistance alleles and within-host diversity for molecular surveillance.
- It is a cost-efficient tool for monitoring emerging drug resistance in malaria parasites.