Single-cell epigenetic landscape, microenvironment interactions, and gene regulatory modules of non-functioning pituitary adenomas - PubMed
7 hours ago
- #tumor microenvironment
- #epigenetics
- #gene regulation
- Study focuses on single-cell epigenetic landscape and tumor microenvironment (TME) interactions in non-functioning pituitary adenomas (NFPAs).
- Single-nucleus multiomics assays conducted on 4 gonadotrope NFPAs (34,819 cells) and 11 non-diseased postmortem control pituitaries (51,535 cells).
- Findings include decreased proportions of tumor-associated endothelial cells and pericytes, and increased proportions of macrophages.
- Identified bidirectional tumor-macrophage crosstalk with nine ligand-receptor interactions, including validated SFRP1-FZD6 interaction linked to tumorigenesis.
- Coordinated gene expression and chromatin accessibility programs distinguished adenoma cells from gonadotropes.
- Gene regulatory circuits (GRCs) altered in adenoma cells, regulated by transcription factors like PBX3 and MEF2C.
- Data available at https://rstudio-connect.hpc.mssm.edu/nfpa_browser/.