Structural conservation and ion selectivity adaptation of the mechanically activated PIEZO channel - PubMed
6 hours ago
- #structural evolution
- #PIEZO channel
- #ion selectivity
- Drosophila PIEZO (dPIEZO) shows unusual ion selectivity for both Cl- and Ca2+, differing from the cation-selective mouse PIEZO1.
- The cryo-EM structure of dPIEZO has a three-bladed propeller shape like mPIEZO1 but is in a distinct conformational state.
- The lateral-plug domain and specific residues (His1391 and Arg1396) determine dPIEZO's ion selectivity; His1391 is conserved from insects to mammals and enables pH-dependent tuning.
- Evolution of Arg1396 to His in mammalian PIEZO1 may transform the lateral-plug domain from a selectivity filter to a pluggable gate.
- The study illuminates the evolutionary conservation, adaptation, and regulation of structure-function properties in PIEZO channels.