Decoding the role of macrophage LAP3 in lung cancer - integration of single-cell technologies and machine learning reveals an orchestrating immunometabolic circuit at the tumor-epithelial interface -
6 hours ago
- #immunometabolism
- #NSCLC
- #LAP3
- Amino acid metabolism is crucial in tumor-immune interactions in non-small cell lung cancer (NSCLC).
- Single-cell RNA-seq and spatial transcriptomics were used to map immunometabolic architecture in NSCLC.
- LAP3 was found to be enriched in tumor epithelium and a specific macrophage subset, localized at epithelial-myeloid interfaces.
- LAP3 overexpression in A549 and PC9 cell lines suppressed proliferation, motility, and invasiveness in vitro.
- In vivo, LAP3-overexpressing xenografts formed smaller tumors in nude mice.
- LAP3 links amino acid catabolism to immune communication, suggesting a dual role in restraining tumor aggressiveness and fostering an immune-responsive microenvironment.
- LAP3 is a potential biomarker for patient stratification and combining metabolic modulation with immunotherapy.