Meiosis-specific protein co-expression in Arabidopsis thaliana: a protein delivery tool in meiotic cells - PubMed
5 hours ago
- #Arabidopsis-thaliana
- #meiosis
- #protein-co-expression
- Meiosis-specific protein co-expression tool developed in Arabidopsis thaliana.
- Utilizes ribosome-skipping peptides (P2a, IntF2a) for co-expression from single polyprotein precursors.
- IntF2a shows more efficient cleavage in male meiocytes compared to P2a.
- Enables synchronized co-expression of up to three proteins during meiosis.
- Applied in studying spatiotemporal dynamics of proteins like H2B, CENH3, and TUA6 with fluorescent proteins.
- Offers potential for modifying meiotic processes and biotechnological applications requiring coordinated protein synthesis.