Selector adeno-associated viral vectors facilitate on-target precise genome editing and purge off-target chromosomal insertions - PubMed
6 hours ago
- #AAV Vectors
- #Precision Genome Editing
- #Off-Target Purging
- Selector adeno-associated viral (AAV) vectors with marker-free selectable sequences enable enrichment for cells with precise on-target edits at endogenous alleles.
- These vectors introduce ATP1A1 polymorphisms conferring resistance to ouabain, achieving high frequencies of accurately edited cell populations.
- Selector AAV vectors effectively purge heterogeneous off-target DNA insertions that arise from conventional AAV-based genome editing methods.
- The approach mitigates issues like off-target chromosomal insertions via nonhomologous end joining and imprecise on-target edits from end-joining pathways.