Timing Mechanotransduction: Mechanically Dynamic Biomaterials Reveal the Temporal Hierarchy of YAP/TAZ Control Nodes - PubMed
6 hours ago
- #dynamic hydrogels
- #YAP/TAZ
- #mechanotransduction
- Development of dynamically softening polyacrylamide hydrogels to study mechanotransduction.
- YAP/TAZ inactivation occurs at an early stiffness threshold, linked to nucleo-cytoskeletal collapse.
- Peripheral focal adhesions remain resilient and undergo centripetal remodeling.
- SUN2 disruption lowers the mechanosensitive threshold; increased contractility raises it.
- Early YAP/TAZ shutoff coincides with microtubule reorganization and AMOT accumulation.
- Nuclear flattening, cell rounding, and peripheral adhesion collapse occur later at lower stiffness thresholds.
- Mechanotransduction is directionally asymmetric, with YAP/TAZ reactivation requiring cyclic strain.
- Findings provide a spatiotemporal framework for dynamic mechanotransduction.