AlphaFold 3-powered discovery of phage proteins that inhibit bacterial transcription initiation - PubMed
6 hours ago
- #AlphaFold 3
- #RNA polymerase
- #transcription regulation
- AlphaFold 3 was used to discover phage proteins that inhibit bacterial transcription initiation.
- Traditional methods for identifying such proteins are labor-intensive and require live phages.
- A new pipeline was developed for AlphaFold 3-guided discovery of these phage proteins.
- Three phage proteins were identified and characterized, each binding to different sites on RNA polymerase.
- These proteins inhibit transcription initiation via distinct mechanisms.
- The pipeline can be adapted to screen modulators of other large protein complexes like ribosomes and CRISPR-Cas systems.