A universal light-controlled highly sensitive one-pot CRISPR/Cas12a diagnostic based on structure-engineered crRNA - PubMed
5 hours ago
- #Light-Controlled Assay
- #CRISPR Diagnostics
- #One-Pot Detection
- ULTRAt is a universal light-controlled one-pot CRISPR/Cas12a diagnostic using structure-engineered crRNA for enhanced sensitivity.
- Photocaged groups in crRNA suppress Cas12a during isothermal amplification; UV irradiation restores activity for robust detection.
- Achieves a detection limit of two copies of monkeypox virus per reaction with results in 15 minutes, 100x more sensitive than conventional methods.
- Supports SNP discrimination and HPV 16/18 genotyping, with high concordance in clinical samples compared to qPCR and sequencing.
- Enables rapid, ultra-sensitive point-of-care diagnostics and versatile genotyping applications.