A Mechanotransduction-Aware Strategy for Enhancing MSC Potency via 3D Culture and Localized Delivery - PubMed
6 hours ago
- #Mesenchymal Stem Cells
- #Acute Lung Injury
- #3D Cell Culture
- A composite system, GelMA@hMSCs-Alg-RGD, was engineered to enhance MSC potency via 3D culture and localized delivery for treating acute lung injury (ALI).
- The design uses RGD-functionalized alginate microbeads to support human MSCs, encapsulated in a dopamine-modified GelMA hydrogel, creating a low-tension niche that preserves MSC identity and boosts paracrine effects.
- Conditioned media from this system promoted endothelial cell functions and suppressed oxidative stress, while inhibiting myofibroblast differentiation in fibroblasts by affecting cytoskeletal dynamics.
- In vivo, the composite enabled prolonged pulmonary retention, reduced inflammation and oxidative stress, improved survival, and promoted macrophage M2 polarization in a murine ALI model.
- This strategy integrates localized retention with paracrine amplification to reprogram immune and mechanical microenvironments, offering a platform for MSC-based regenerative medicine.