Limitations of serial cloning in mammals - PubMed
5 hours ago
- #genetic mutations
- #mammals
- #serial cloning
- Serial cloning in mammals was conducted for 20 years from a single donor mouse.
- Re-cloned mice appeared normal with normal lifespans but accumulated large structural and lethal DNA mutations with each generation.
- Birth rate of serial cloning declined from the 27th generation, with the 58th generation being the last.
- Oocytes from near-final generation re-cloned mice could be fertilized, but most embryos degenerated.
- A few embryos normalized by meiosis and fertilization developed to full term, indicating sexual reproduction is essential for eliminating genetic anomalies from clonal reproduction.