Bifidobacterium intestinale sp. nov., and Blautia caeci sp. nov., from the Human Gut Microbiome with Candidate Probiotic Potential - PubMed
2 hours ago
- #gut microbiome
- #probiotic potential
- #novel bacterial species
- Two novel bacterial strains, M3-R-103T and C3-R-101T, were isolated from human fecal samples, with M3-R-103T identified as belonging to the genus Bifidobacterium and C3-R-101T to Blautia, based on phylogenetic analysis using 16S rRNA gene sequences.
- Strain M3-R-103T shows 98.9% 16S rRNA gene similarity to Bifidobacterium pseudocatenulatum DSM 20438T, while C3-R-101T shows 98.6% similarity to Blautia hominis KB1T. Their draft genomes are 2.08 Mbp and 6.40 Mbp in size, with DNA G+C contents of 56.3 mol% and 46.7 mol%, respectively.
- Both strains exhibit tolerance to gastrointestinal stress conditions, including acidic pH (2.0) and bile salts (0.3%), and demonstrate in vitro antioxidant activity (DPPH radical scavenging from 38.28% to 58.83%), α-amylase inhibition (from 47.12% to 69.19%), and anti-inflammatory effects (inhibition of albumin denaturation from 52.99% to 58.16%).
- Based on combined phylogenetic, genomic, and phenotypic evidence, the strains are proposed as novel species: Bifidobacterium intestinale sp. nov. (type strain M3-R-103T) and Blautia caeci sp. nov. (type strain C3-R-101T), with potential probiotic properties.