Thymidine-Auxotrophic Salmonella Enables Selective Tumor Colonization and Adenosine Depletion for Cancer Immunotherapy - PubMed
5 hours ago
- #engineered bacteria
- #adenosine depletion
- #cancer immunotherapy
- Engineered thymidine-auxotrophic Salmonella (TK VNP) selectively targets tumors due to high dTMP levels in the tumor microenvironment.
- TK VNP enhances safety and tumor-targeting efficiency by nearly tenfold compared to the parental strain.
- Adenosine deaminase (ADD) modules (cTKA VNP, sTKA VNP, wTKA VNP) degrade immunosuppressive adenosine, boosting CD8+ T cell activity.
- TKA VNP reduces T cell exhaustion markers (PD-1, TIM-3) and synergizes with immune checkpoint blockade and radiotherapy.
- This platform combines tumor-restricted bacterial replication with localized immunometabolic modulation for safer, effective cancer therapy.