From Image to Insight: Optimized Multiplex Staining Panel for FFPE Mouse Bone Marrow Enables Spatial Profiling of the Hematopoietic Niche - PubMed
5 hours ago
- #Spatial profiling
- #Bone marrow microenvironment
- #Multiplex Immunohistochemistry
- Cellular composition and spatial architecture of the bone marrow niche are critical for regulating normal and malignant hematopoiesis.
- Flow cytometry analyzes hematopoietic and niche cell composition but disrupts spatial context due to single-cell dissociation.
- Immunofluorescence imaging preserves tissue architecture but is limited in the number of markers that can be detected simultaneously.
- Multiplex staining technologies allow visualization of numerous antigens in a single tissue section, offering a comprehensive view of the bone marrow microenvironment.
- Validated multiplex panels for analyzing murine bone marrow, especially FFPE sections, are limited due to challenges like high autofluorescence and tissue damage during antigen retrieval.
- The study optimized tissue preparation for staining murine FFPE bone marrow and developed an efficient multiplex immunohistochemistry panel for detecting hematopoietic stem cell-containing populations in their native niche architecture.
- Application of the panel to FFPE bone marrow sections from Jak2V617F mice revealed alterations in the megakaryocytic niche, demonstrating its utility for characterizing disease-associated spatial changes.