Interaction Between Rec8 and Mis4 Is Required for Axis-Loop Chromatin Formation and Homologous Chromosome Recombination During Meiosis - PubMed
5 hours ago
- #Chromatin Structure
- #Cohesin
- #Meiosis
- Rec8 cohesin complex is essential for homologous chromosome pairing, recombination, and sister chromatid cohesion during meiosis.
- In fission yeast, a rec8-F204S mutant disrupts axis-loop chromatin formation but retains sister chromatid cohesion, leading to reduced meiotic recombination.
- Mis4 (NIPBL/Scc2) mediates Rec8 cohesin complex loading onto chromatin.
- A mis4-LR mutant (L1150S/R1159G) phenocopies rec8-F204S, impairing chromatin axis formation and homologous recombination while maintaining sister chromatid cohesion.
- Mutation sites (Mis4-L1150, Mis4-R1159, Rec8-F204) are located at the Mis4-Rec8 interaction surface.
- Biochemical analysis shows the Mis4-LR mutant has reduced Rec8-binding activity.
- The Mis4-Rec8 interaction is necessary for proper Rec8-dependent meiotic chromosome axis formation.