Flow cytometry-based monitoring of chimeric antigen receptor (CAR) T cells: Reagent selection, assay design, and clinical utility - PubMed
6 hours ago
- #Clinical utility
- #Flow cytometry
- #CAR T cells
- Accurate quantification of CAR T cells is essential for monitoring post-infusion expansion and persistence.
- Multiparameter flow cytometry (MFC) enables rapid, live-cell detection with absolute quantification and immunophenotypic characterization.
- Review focuses on practical and technical aspects of flow cytometry-based CAR T-cell monitoring.
- Discusses selection of CAR detection reagents (target-specific, construct-specific, and target-agnostic strategies).
- Covers assay optimization, purpose-driven panel design, and validation for peripheral blood and other specimens.
- Addresses assay considerations unique to gene-edited allogeneic CAR T-cell products.
- Discusses the role of MFC in identifying CAR T-cell clonal expansions and evaluating secondary hematolymphoid neoplasms.