CBASS limits bacteriophage production while maintaining cell viability in Pseudomonas aeruginosa - PubMed
5 hours ago
- #bacteriophage
- #Pseudomonas aeruginosa
- #CBASS
- CBASS is an anti-phage immune system that generates cyclic nucleotide signals to activate effectors.
- The phospholipase effector CapV in Pseudomonas aeruginosa, when activated by 3',3'-cGAMP, limits phage production without causing cell death.
- Activation of CapV allows normal cell growth and viability while blocking phage DNA packaging and capsid assembly, preventing phage replication.
- Phage transcription and initial DNA replication proceed normally under CBASS activation, but full DNA levels and packaging are impaired.
- The study suggests CBASS disrupts phage assembly at the inner membrane, enabling anti-viral activity while preserving host viability.