Molecular mechanisms of shenhong tongluo formula against myocardial ischemia-reperfusion injury: Insights from RNA sequencing, bioinformatics, reverse network pharmacology, molecular docking, and mole
6 hours ago
- #cardioprotection
- #ferroptosis
- #traditional Chinese medicine
- The study explores how Shenhong Tongluo Formula (SHTLF) protects against myocardial ischemia-reperfusion injury (MIRI) by inhibiting ferroptosis.
- RNA sequencing and bioinformatics identified SAT1 and PTGS2 as key targets regulated by SHTLF in ferroptosis pathways.
- Reverse network pharmacology pinpointed quercetin as a bioactive compound in SHTLF that binds to SAT1 and PTGS2, supported by molecular docking and dynamics simulations.
- Experimental validation showed that SHTLF suppresses ferroptosis by downregulating the SAT1/ALOX15 axis, reducing lipid peroxidation and cardiomyocyte damage.