Phage-antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance - PubMed
12 hours ago
- #Phage-antibiotic synergy
- #Biofilm eradication
- #Multidrug resistance
- Phage-antibiotic synergy (PAS) enhances β-lactam efficacy against MDR Klebsiella quasipneumoniae biofilms.
- Light-sheet fluorescence microscopy (LSFM) was used for real-time, noninvasive viability analysis of PAS in biofilms.
- Combination of high-dose phages and low-dose ceftazidime (CAZ) significantly reduced biofilm viability compared to mono-treatments.
- Phage coadministration degraded extracellular polymeric substance (EPS) polysaccharides, enhancing antibiotic penetration.
- Phage mono-treatment led to resistant mutants, but combination therapy fully suppressed resistance.
- Mutations in genes like fhuA, purA, and rpoC were linked to resistance mechanisms and fitness trade-offs.
- The study provides a foundation for optimizing PAS-based therapies for device-associated infections.