Activation and regulation of the dynein-dynactin-NuMA complex - PubMed
6 hours ago
- #microtubule dynamics
- #mitotic regulation
- #cell division
- NuMA plays a key role in cell division by focusing microtubule minus-ends and generating cortical force.
- The dynein-dynactin-NuMA complex (DDN) structure was determined using cryo-electron microscopy and in vitro reconstitution.
- NuMA's N terminus facilitates dynein motility and transport, while its C terminus binds and stabilizes microtubule minus-ends.
- Full-length NuMA is autoinhibited but becomes activated through mitotic phosphorylation, enabling dynein function.
- Activated NuMA, together with dynein, organizes microtubule minus-ends into aster-like structures.
- The study provides insights into the regulation of NuMA and dynein during mitosis.