Preservation of chondrocyte microspheroids by local sustained hydrogen supply improves osteoarthritic cartilage repair - PubMed
6 hours ago
- #cartilage repair
- #tissue engineering
- #hydrogen therapy
- The inflammatory microenvironment in osteoarthritis (OA) degrades stem cell grafts, hindering cell therapies.
- Bone marrow-derived mesenchymal stem cell (BMSC)-derived chondrocyte microspheroids (CMSs) maintain viability and hyaline cartilage phenotype with sustained hydrogen (H2) supply during spheroidization and in OA conditions.
- TiSi2 nanosheets (TSN hydrolytically generate high-dose H2 for over two months and are used in a H2-releasing hydrogel transplant (TSN/CMS-Gel) with photo-crosslinking hydrogel (Gel).
- TSN/CMS-Gel transplantation enhances chondrocyte survival in rodent OA models, repairs sheep critical-size cartilage defects, and reverses OA progression within six months.
- Local sustained H2-mediated preservation of transplanted chondrocytes in pathological environments improves cell transplantation outcomes.