Efficient genome editing with chimeric oligonucleotide-directed editing - PubMed
7 hours ago
- #CODE System
- #Genome Editing
- #Prime Editing
- Prime editing is a precise genome editing tool, and the study introduces a new system called chimeric oligonucleotide-directed editing (CODE).
- CODE uses nCas9-DNA polymerase fusion proteins and a chimeric pegRNA for programmable search-and-replace genome editing.
- CODEMax(exo+) variant includes 5' to 3' exonuclease activity to improve strand invasion and repair outcomes.
- CODE demonstrates improved efficiency over PEMax for small insertions, deletions, and substitutions in HEK293T cells.
- CODEMax successfully edits mouse and bovine embryos with up to 9.3% precision.
- CODE expands genome editing tools without creating double-stranded breaks and complements existing prime editors.