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A targeting lentiviral vector for generation of CAR-T cells in vivo - PubMed

5 hours ago
  • #In Vivo Engineering
  • #CAR-T
  • #Lentiviral Vector
  • Development of a T cell-specific lentiviral vector for in vivo CAR-T cell generation.
  • The lentiviral vector uses an engineered Sindbis virus envelope to target human T cells specifically, without affecting other immune cells.
  • No additional anti-CD3/CD28 stimulation is required for primary T cell activation during in vitro infection.
  • Successful delivery of a CD19-targeting CAR molecule to human primary T cells in vivo.
  • In vivo generated CD19-CAR-T cells effectively cleared B cell lymphoma.
  • Potential to simplify CAR-T cell therapy by eliminating the need for ex vivo T cell engineering.